β-glukosidase

23 Feb

β-glukosidase adalah enzim glukosidase yang memutuskan ikatan β(1->4) glikosida antara 2 glukosa atau molekul pengganti glukosa yang lain seperti selobiosa. Enzim ini bertindak sebagai katalis dalam proses hidrolisis residu ujung non pereduksi pada β-D-Glukosa, dengan melepaskan unit glukosa. Selulosa adalah komponen utama yang memiliki polimer ikatan β antara molekul glukosa didalamnya, dan enzim ini dibutuhkan oleh organisme tertentu seperti jamur, bakteri, dan rayap untuk bisa mengkonsumsi selulosa tersebut.

Enzim β-glukosidase (BGL, EC 3.2.1.21) merupakan salah satu enzim dari sistem enzim selulase, selain endo-1,4-β-glukanase (1,4-β-D-glucan 4-glucanohydrolase, EC 3.2.1.4. cellulase) dan selobiohidrolase (1,4-β-D-glucan cellobiohydrolase, EC 3.2.1.91, cellulase 1,4-β-cellobiosidase). β-glukosidase diproduksi oleh berbagai macam jamur ataupun bakteri, antara lain Trichoderma reesei, Acetobacter xylinum, Cladosporium resinae, Trichoderma viride, Pennicilium janthinellum, Flavobacterium johnsonae, Paecylomyces bainier, Phanerochaete chrysosporium, Pyrococus furiosus, Cellvibrio gilvus, dan Aspergillus sp.

β-glukosidase dari berbagai sumber juga telah diisolasi, dimurnikan, dikarakterisasi, dan dipelajari sifat-sifatnya, diantaranya β-glukosidase dari Saccharomyces lactis strain Y-123 (Marchin & Duerksen 1968), Flavobacterium M64 (Sano, Amemura & Harada 1975), Bacillus circulans subsp. alkalophilus (Paavilainen, Hellman & Korpela 1993), Aureobasidium pullulans (Saha, Freer & Bothast 1994), Xylaria regalis (Wei et al. 1996), Humicola grisea (Takashima et al. 1999), Acetobacter xylinum (Tajima et al. 2001), Thermoascus aurantiacus (de Palma-Fernandez, Gomes & da Silva 2002), dan Fomitopsis palustris (Yoon, Kim & Cha 2008).

*sumber :

Marchin, G.L. & Duerksen, J.D., 1968. Purification of beta-glucosidase from Saccharomyces lactis strain Y-123. Journal of Bacteriology, 96(4), 1181-6.

Sano, K., Amemura, A. & Harada, T., 1975. Purification and properties of a beta-1,6-clucosidase from Flavobacterium. Biochimica Et Biophysica Acta, 377(2), 410-20.

Paavilainen, S., Hellman, J. & Korpela, T., 1993. Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus. Applied and Environmental Microbiology, 59(3), 927-32.

Saha, B.C., Freer, S.N. & Bothast, R.J., 1994. Production, Purification, and Properties of a Thermostable {beta}-Glucosidase from a Color Variant Strain of Aureobasidium pullulans. Appl. Environ. Microbiol., 60(10), 3774-3780.

Wei, D. et al., 1996. Purification and Characterization of an Extracellular β-Glucosidase from the Wood-Grown Fungus Xylaria regalis. Current Microbiology, 33(5), 297-301.

Tajima, K. et al., 2001. Cloning and sequencing of the beta-glucosidase gene from Acetobacter xylinum ATCC 23769. DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes, 8(6), 263-9.

Takashima, S. et al., 1999. Molecular Cloning and Expression of the Novel Fungal {beta}-Glucosidase Genes from Humicola grisea and Trichoderma reesei. J Biochem, 125(4), 728-736.

de Palma-Fernandez, E., Gomes, E. & da Silva, R., 2002. Purification and characterization of two β-glucosidases from the thermophilic fungus Thermoascus aurantiacus. Folia Microbiologica, 47(6), 685-690.

Yoon, J., Kim, K. & Cha, C., 2008. Purification and characterization of thermostable β-glucosidase from the brown-rot basidiomycete Fomitopsis palustris grown on microcrystalline cellulose. The Journal of Microbiology, 46(1), 51-55.<!–[if supportFields]><![endif]–>


2 Responses to “β-glukosidase”

  1. malicemrc October 17, 2009 at 5:31 pm #

    hmm? ko gabisa dibuka link-nya?

  2. mediccall June 7, 2009 at 6:42 am #

    I read and consult Biophysica Curse and Fundaments is a medical textbook that provides a comprehensive overview of Biophysica (download PDF). has also been published in English , Spanish ,French Albanian, Chinese, Croatian, German, Indonesian, Italian, Mongolian, Serbian, and Slovenian.
    http://mediccall.blogspot.com/search/label/Biophysica

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